PUBLICATIONS

Representative Publications


Chen, Y. G., A. Mantalaris, P. Bourne, P. Keng, J. H. D. Wu (2000) Expression of mPer1 and mPer2, two mammalian clock genes, in murine bone marrow. Biochem. Biophy. Res. Commun. 26:724-728.

Mantalaris, A., P. Keng, P. Bourne, A.Y.C. Chang, and J.H.D. Wu (1998) Engineering a human bone marrow model: a case study on ex vivo erythropoiesis. Biotechnology Progress 14:126-133.

Wang, T.Y., J.K. Brennan, and J.H.D. Wu (1995) Multilineal hemopoiesis in a three-dimensional murine long-term bone marrow culture. Exp. Hematol. 23:26-32.

Lytle, B., B.F. Volkman, W.M. Westler, M. P. Heckman, and J.H.D. Wu (2001) Solution structure of a type I dockerin domain, a novel prokaryotic, extracellular calcium-binding domain. J. Mol. Biol. 307:745-753.

Kruus, K., A.C. Lua, A.L. Demain, and J.H.D. Wu (1995) The anchorage function of CipA (CelL), a scaffolding protein of the Clostridium thermocellum cellulosome. Proc. Natl. Acad. Sci. USA 92:9254-9258.

Wang, W.K., K. Kruus, and J.H.D. Wu (1993) Cloning and the DNA sequence of the gene coding for the Clostridium thermocellum cellulase SS, a major cellulosome subunit. J. Bacteriol. 175:1293-1302.


Bone Marrow Tissue Engineering and Hematopoiesis/Lymphopoiesis


Waugh, R.E., A. Mantalaris, R.G. Bauserman, W.C. Hwang, and J.H.D. Wu (2001) Membrane instability in late stage erythropoiesis. Blood 97:1869-1875.

Omasa, T., Y.G. Chen, A. Mantalaris, and J. H. D. Wu (2001) A cDNA from human bone marrow encoding a protein exhibiting homology to the ATP1?1/PLM/MAT8 family of transmembrane proteins. Biochim. Biophys. Acta 1517:307-310.

Mantalaris, A., N. Panoskaltsis, Y. Sakai, P. Bourne, C.S. Chang, E.M. Messing, and J.H.D. Wu (2001) Localization of androgen receptor expression in human bone marrow. J. Pathol. 193:361-366.

Chen, Y. G., A. Mantalaris, P. Bourne, P. Keng, J. H. D. Wu (2000) Expression of mPer1 and mPer2, two mammalian clock genes, in murine bone marrow. Biochem. Biophy. Res. Commun. 26:724-728.

Mantalaris, A., P. Keng, P. Bourne, A.Y.C. Chang, and J.H.D. Wu (1998) Engineering a human bone marrow model: a case study on ex vivo erythropoiesis. Biotechnology Progress 14:126-133.

Huang, L.H., and J.H.D. Wu (1997) Production of colony-stimulating activity by a murine bone marrow stromal cell line on porous microcarriers. Biotechnology Techniques 11:311-314.

Huang, L.H., and J.H.D. Wu (1997) Cell density-dependent proliferation of the murine bone marrow-derived stromal cell lines. Biotechnol. Lett. 19:89-92.

Felten, D., K. Gibson-Berry, and J.H.D. Wu (1996) Innervation of bone marrow by tyrosine hydroxylase-immunoreactive nerve fibers and hemopoiesis-modulating activity of a -adrenergic agonist in mouse. Mole. Biol. Hematopoiesis 5:627-636.

Wang, T.Y., J.K. Brennan, and J.H.D. Wu (1995) Multilineal hemopoiesis in a three-dimensional murine long-term bone marrow culture. Exp. Hematol. 23:26-32.

Wang, T.Y., A. Mantalaris, and J.H.D. Wu (1994) A novel three-dimensional long-term bone marrow bioreactor culture system. IN: T. Kobayashi, Y. Kitagawa, and K. Okumura (editors), Animal Cell Technology: Basic & Applied Aspects, Vol. 6, pp115-120. Dordrecht: Kluwer Academic Publishers.

Wang, T.Y., and J.H.D. Wu (1992) A continuous perfusion bioreactor for long-term bone marrow culture. Ann. N.Y. Acad. Sci. 665:274-284.


Cellulase Molecular Enzymology and Structure



Lytle, B., B.F. Volkman, W.M. Westler, M. P. Heckman, and J.H.D. Wu (2001) Solution structure of a type I dockerin domain, a novel prokaryotic, extracellular calcium-binding domain. J. Mol. Biol. 307:745-753.

Lytle, B., B.F. Volkman, W.M. Westler, and J.H.D. Wu (2000) Secondary structure and calcium-induced folding of the Clostridium thermocellum dockerin domain determined by NMR spectroscopy. Arch. Biochem. Biophy. 379:237-244.

Lytle, B., W.M. Westler, and J.H.D. Wu (1999) Molecular assembly of the Clostridium thermocellum cellulosome. IN: K. Ohmiya, K. Hayashi, K. Sakka, Y. Kobayashi, S. Karita, and S. Kimura (editors), Genetics, Biochemistry, and Ecology of Cellulose Degradation, pp444-449. Tokyo: Uni Publishers.

Lytle, B., and J.H.D. Wu (1998) Involvement of both dockerin calcium-binding subdomains in the assembly of the Clostridium thermocellum cellulosome. J. Bacteriol. 180:6581-6585.

Fukumura, M., A. Begum, K. Kruus, and J.H.D. Wu (1997) Interactions and synergism between the recombinant CelD, an endoglucanase, and the Cellulosome-Integrating Protein (CipA) of Clostridium thermocellum. J. Ferment. Bioeng. 83:146-151.

Lytle, B., C. Myers, K. Kruus, and J.H.D. Wu (1996) Interactions of the CelS binding ligand with various receptor domains of the Clostridium thermocellum cellulosomal scaffolding protein, CipA. J. Bacteriol. 178:1200-1203.

Kruus, K., A. Andreacchi, W.K. Wang, and J.H.D. Wu (1995) Product inhibition of the recombinant CelS, a major cellulosome component and exoglucanase of Clostridium thermocellum. Appl. Microbiol. Biotechnol. 44:399-404.

Mantalaris, A., and J.H.D. Wu (1995) Developing an ex vivo mimic of bone marrow: a three-dimensional bone marrow bioreactor. Bulletin of Intensive and Critical Care 2:12-15.

Kruus, K., A.C. Lua, A.L. Demain, and J.H.D. Wu (1995) The anchorage function of CipA (CelL), a scaffolding protein of the Clostridium thermocellum cellulosome. Proc. Natl. Acad. Sci. USA 92:9254-9258.

Kruus, K., W.K. Wang, J. Ching, and J.H.D. Wu (1995) Exoglucanase activities of the recombinant Clostridium thermocellum CelS, a major cellulosome component. J. Bacteriol. 177:1641-1644.

Wang, W.K., K. Kruus, and J.H.D. Wu (1994) Cloning and expression the Clostridium thermocellum celS gene in Escherichia coli. Appl. Microbiol. Biotechnol. 42:346-352.

Kruus, K., W.K. Wang, P.-C. Chiu, J. Ching, T,-Y. Wang, and J.H.D. Wu (1994) CelS: a major exoglucanase component of the Clostridium thermocellum cellulosome. ACS Symp. Ser. 566:84-99.

Wang, W.K., K. Kruus, J. Ching, and J.H.D. Wu (1994) The mechanism of crystalline cellulose degradation by the Clostridium thermocellum cellulase complex. IN: K. Shimada, S. Hoshino, K. Ohmiya, K. Sakka, Y. Kobayashi, and S. Karita (editors), Genetics, Biochemistry, and Ecology of Lignocellulose Degradation, pp23-31. Tokyo: Uni Publishers.

Wang, W.K., K. Kruus, and J.H.D. Wu (1993) Cloning and the DNA sequence of the gene coding for the Clostridium thermocellum cellulase SS, a major cellulosome subunit. J. Bacteriol. 175:1293-1302.

Wang, W.K., and J.H.D. Wu (1993) Structural features of the Clostridium thermocellum cellulase SS gene. Appl. Biochem. Biotechnol. 39/40:149-158.

Wu, J.H.D. (1993) The Clostridium thermocellum cellulosome: A new mechanistic concept for cellulose degradation. ACS Symp. Ser. 516:251-264.

Demain, A.L., and J.H.D. Wu (1989) The cellulase complex of Clostridium thermocellum. IN: T.K. Ghose (editor), Bioprocess Engineering, pp68-86. Chichester: Ellis Horwood.

Wu, J.H.D., and A.L. Demain (1988) Proteins of the Clostridium thermocellum cellulase complex responsible for degradation of crystalline cellulose. FEMS Symp. 43:117-131.

Wu, J.H.D., W.H. Orme-Johnson and A.L. Demain (1988) Two components of an extracellular protein aggregate of Clostridium thermocellum together degrade crystalline cellulose. Biochemistry 27:1703-1709.


Fermentation Microbiology


Andreacchi, A.S., T. Wang, and J.H.D. Wu (1997) Vascular effect of the fungal extract of Basidiomycete species YL 8006. Life Sciences 60:1987-1994.

Wu, J.H.D., L. Yang, and A.L. Demain (1984) Further study on the role of phenylalanine in Gramicidin S biosynthesis by Bacillus brevis. J. Biotechnol. 1:81-94.

Wu, J.H.D., and A.L. Demain (1984) Glutamine does not repress Gramicidin S biosynthesis
in Bacillus brevis. J. Ferment. Technol. 62:389-391.

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